山羊颞下颌关节盘胶原构筑和生物力学性能研究
目的为工程化颞下颌关节盘组织建立客观的生物力学标准。方法从4只健康1月龄山羊头中完整分离8个颞下颌关节盘，用直径3mm的冲子在前、中、后带取圆柱状试样，预处理压缩速度为0．025mm·s-1，结束条件为最大载荷小于4．9N，压缩位移1．5mm，循环3次，在预处理基础上完成压缩实验，获取各区带的应力应变关系图和弹性模量值。扫描电子显微镜和偏振光显微镜观察各区带的超微结构和胶原纤维走向。结果1）关节盘组织的生理应变范围在10％以内，关节盘组织的拟线性本构方程可以用多项式y=ax＋bx2＋cx，进行拟合。2）关节盘中间带的弹性模量值最大，其与前带和后带均有统计学差异（P〈0．05），前带与后带之间无...
Saved in:
| Published in: | 华西口腔医学杂志 Vol. 34; no. 1; pp. 73 - 77 |
|---|---|
| Main Author: | |
| Format: | Journal Article |
| Language: | Chinese |
| Published: |
西北民族大学口腔医学国家民委重点实验室，兰州 730030%兰州大学生命科学学院,兰州,730000
2016
兰州大学口腔医学研究所,兰州,730000%兰州大学口腔医学研究所，兰州 730000 |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | 目的为工程化颞下颌关节盘组织建立客观的生物力学标准。方法从4只健康1月龄山羊头中完整分离8个颞下颌关节盘，用直径3mm的冲子在前、中、后带取圆柱状试样，预处理压缩速度为0．025mm·s-1，结束条件为最大载荷小于4．9N，压缩位移1．5mm，循环3次，在预处理基础上完成压缩实验，获取各区带的应力应变关系图和弹性模量值。扫描电子显微镜和偏振光显微镜观察各区带的超微结构和胶原纤维走向。结果1）关节盘组织的生理应变范围在10％以内，关节盘组织的拟线性本构方程可以用多项式y=ax＋bx2＋cx，进行拟合。2）关节盘中间带的弹性模量值最大，其与前带和后带均有统计学差异（P〈0．05），前带与后带之间无统计学差异（P=0．361）。3）颞下颌关节盘内部呈现环形胶原网状结构，中间带胶原排列致密，呈前后向排列；前带和后带主要呈内外走向，伴有前后方向的纤维。结论环形胶原网状结构是山羊颞下颌关节盘抗力性能的基础，中间带抗压性能高于前带和后带。 |
|---|---|
| Bibliography: | 51-1169/R temporomandibular joint disc; quasi linearconstitution equation; compressive testing; elastic modu-lus; scanning electron microscope; polarized light mi-croscope Objective To establish objectively biomechanical criteria for temporomandibular joint disc tissue engineerings. Methods Eight temporomandibular joint discs from a four-month-old goat were separated completely. A cylindrical sample with diameter of 3 mm in the anterior, intermediate, and posterior bands was obtained, and the samples underwent pre-com- pression test with three cycles under a speed of 0.025 mm.s-l. With the end condition for the maximum load of less than 4.9 N and 1.5 mm compression displacement, the formal compression test was performed with the same speed. Moreover, this test obtained the stress-strain relationship and elastic modulus of each disc band. Ultrastructure and collagen fiber orientation of the district zone were observed by scanning electron microscope and polarized light microscope. Results 1） The physiological st |
| ISSN: | 1000-1182 |
| DOI: | 10.7518/hxkq.2016.01.015 |