Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform
In this study, the 454 pyrosequencing technology was used to analyze the DNA of the Microcystis aeruginosa symbiosis system from cyanobacterial algal blooms in Taihu Lake, China. We generated 183 228 reads with an average length of 248 bp. Running the 454 assembly algorithm over our sequences yielde...
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| Published in: | Chinese journal of oceanology and limnology Vol. 29; no. 3; pp. 505 - 513 |
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| Main Author: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
Heidelberg
SP Science Press
01-05-2011
Springer Nature B.V |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | In this study, the 454 pyrosequencing technology was used to analyze the DNA of the Microcystis aeruginosa symbiosis system from cyanobacterial algal blooms in Taihu Lake, China. We generated 183 228 reads with an average length of 248 bp. Running the 454 assembly algorithm over our sequences yielded 22 239 significant contigs. After excluding the M. aeruginosa sequences, we obtained 1 322 assembled contigs longer than 1 000 bp. Taxonomic analysis indicated that four kingdoms were represented in the community： Archaea （n=9; 0.01%）, Bacteria （n=98 921; 99.6%）, Eukaryota （n=373; 3.7%）, and Viruses （n=18; 0.02%）. The bacterial sequences were predominantly Alphaproteobacteria （n=41 805; 83.3%）, Betaproteobacteria （n=5 254; 10.5%） and Gammaproteobacteria （n= 1 180; 2.4%）. Gene annotations and assignment of COG （clusters of orthologous groups） functional categories indicate that a large number of the predicted genes are involved in metabolic, genetic, and environmental information processes. Our results demonstrate the extraordinary diversity of a microbial community in an ectosymbiotic system and further establish the tremendous utility of pyrosequencing. |
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| Bibliography: | LI Nan , ZHANG Lei, LI Fuchao , WANG Yuezhu , ZHU Yongqiang, KANG Hui, WANG Shengyue, QIN Song （1 Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China 2 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 3 Graduate University of Chinese Academy of Sciences, Beijing 100049, China 4 South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangdong 510301, China 5 Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai 201203, China） In this study, the 454 pyrosequencing technology was used to analyze the DNA of the Microcystis aeruginosa symbiosis system from cyanobacterial algal blooms in Taihu Lake, China. We generated 183 228 reads with an average length of 248 bp. Running the 454 assembly algorithm over our sequences yielded 22 239 significant contigs. After excluding the M. aeruginosa sequences, we obtained 1 322 assembled contigs longer than 1 000 bp. Taxonomic analysis indicated that four kingdoms were represented in the community： Archaea （n=9; 0.01%）, Bacteria （n=98 921; 99.6%）, Eukaryota （n=373; 3.7%）, and Viruses （n=18; 0.02%）. The bacterial sequences were predominantly Alphaproteobacteria （n=41 805; 83.3%）, Betaproteobacteria （n=5 254; 10.5%） and Gammaproteobacteria （n= 1 180; 2.4%）. Gene annotations and assignment of COG （clusters of orthologous groups） functional categories indicate that a large number of the predicted genes are involved in metabolic, genetic, and environmental information processes. Our results demonstrate the extraordinary diversity of a microbial community in an ectosymbiotic system and further establish the tremendous utility of pyrosequencing. 37-1150/P Microcystis aeruginosa; ectosymbiosis; diversity; COGs; algal bloom; metagenome ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0254-4059 2096-5508 1993-5005 2523-3521 |
| DOI: | 10.1007/s00343-011-0056-0 |