一株抗镉真菌的分类学鉴定和镉吸附

一株抗镉真菌的分类学鉴定和镉吸附

研究一株高抗镉真菌M1的分类学鉴定和镉吸附影响因素。通过分子生物学方法获得ITS 和β-微管蛋白的基因序列，并进行测序和分析；采用摇瓶振荡培养方法研究影响镉生物吸附能力的因素；采用傅里叶红外光谱对菌丝的镉吸附机理进行初步分析。基因序列相似性比较和系统发育树构建均表明该真菌与淡紫拟青霉的关系最近，表明真菌M1在分类学上属于淡紫拟青霉菌。活的和失活菌丝体分别在起始pH值为6-7和5-7的范围内表现出较好的吸附能力，镉溶液浓度为100 mg/L 时吸附效率最好。锌和锰对菌体的镉吸附有些许影响，而铜和铅对它的镉吸附影响明显。红外光谱分析表明：菌体吸附镉主要与羰基、羟基和氨基等基团有关。实验结果表明：真...

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Bibliographic Details
Published in:中国有色金属学报：英文版 no. 9; pp. 2759 - 2765
Main Author: 曾晓希 柴立元 汤建新 刘学端 杨志辉
Format: Journal Article
Language:English
Published: 2013
Subjects:
傅里叶红外光谱分析
分类学鉴定
淡紫拟青霉
镉吸附
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Summary:研究一株高抗镉真菌M1的分类学鉴定和镉吸附影响因素。通过分子生物学方法获得ITS 和β-微管蛋白的基因序列，并进行测序和分析；采用摇瓶振荡培养方法研究影响镉生物吸附能力的因素；采用傅里叶红外光谱对菌丝的镉吸附机理进行初步分析。基因序列相似性比较和系统发育树构建均表明该真菌与淡紫拟青霉的关系最近，表明真菌M1在分类学上属于淡紫拟青霉菌。活的和失活菌丝体分别在起始pH值为6-7和5-7的范围内表现出较好的吸附能力，镉溶液浓度为100 mg/L 时吸附效率最好。锌和锰对菌体的镉吸附有些许影响，而铜和铅对它的镉吸附影响明显。红外光谱分析表明：菌体吸附镉主要与羰基、羟基和氨基等基团有关。实验结果表明：真菌M1在重金属的生物修复中具有较好的应用前景。
Bibliography:43-1239/TG
taxonomy characterization; cadmium biosorption; Paecilomyces lilacinu; Fourier transform infrared spectroscopy
Xiao-xi ZENG, Li-yuan CHAI, Jian-xin TANG, Xue-duan LIU, Zhi-hui YANG（ 1. School of Metallurgy and Environment, Central South University, Changsha 410083, China; 2. Hunan Key Laboratory of Green Packaging and Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China; 3. School of Mineral Processing and Bioengineering, Central South University, Changsha 410083, China）
The taxonomy characteriazation and cadmium （Cd） biosorption of the high Cd-resistant fungus M1 were investigated. The internal transcribed spacers （ITS） region and β-tubulin genes of the strain were amplified, sequenced and analyzed by molecular biology technology. The Cd biosorption assay was performed by shaking flask. Fourier transform infrared spectroscopy was used to analyze the mycelium. The similarity of gene sequences and phylogenetic trees show the very close relation between the strain and Paecilomyces lilacinus, and the fungus M1 was identified as P. Lilacinus. The initial pH 6 and Cd concentration about 100 mg/L are optimum. Zn and Mn have a little effect on the Cd biosorption of the strain, while Cu and Pb present obvious effects. FTIR analysis shows that the fungus adsorbs Cd by esters, anhydride, and amide. With the preferable absorption capacity, fungus M1 is considered to have good prospects in bioremediation.
ISSN:1003-6326