人参皂甙Rg3对高糖条件下人视网膜血管内皮细胞的作用研究

人参皂甙Rg3对高糖条件下人视网膜血管内皮细胞的作用研究

目的：观察正常和高糖条件下人参皂甙Rg3对人视网膜血管内皮细胞血管新生作用的影响。方法：在正常和高糖条件的培养基中,分别加入0.1mmol/L和0.5mmol/L的人参皂甙Rg3,在24h,48h和72h用MTT检测细胞的增殖情况,用Transwell小室检测细胞迁徙情况,用Matrigel检测细胞管腔形成的情况,用实时定量RT-PCR和Western-Blot检测细胞中血管内皮生长因子mRNA和蛋白的表达情况。结果：在正常和高糖条件下,人参皂甙Rg3对于人视网膜血管内皮细胞增殖、细胞迁徙和细胞内皮管腔形成均具有抑制作用,且呈浓度与时间依赖性;且人参皂甙Rg3具有抑制人视网膜血管内皮细胞VEG...

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Bibliographic Details
Published in:国际眼科杂志 Vol. 13; no. 10; pp. 1965 - 1969
Main Author: 曹亮 宋愈 吴莹 黄黎黎
Format: Journal Article
Language:Chinese
Published: 中国江苏省南通市第一人民医院眼科,226000 2013
Subjects:
人参皂甙Rg3
血管内皮生长因子
视网膜血管内皮细胞
血管内皮生长因子
人参皂甙Rg3
视网膜血管内皮细胞
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Summary:目的：观察正常和高糖条件下人参皂甙Rg3对人视网膜血管内皮细胞血管新生作用的影响。方法：在正常和高糖条件的培养基中,分别加入0.1mmol/L和0.5mmol/L的人参皂甙Rg3,在24h,48h和72h用MTT检测细胞的增殖情况,用Transwell小室检测细胞迁徙情况,用Matrigel检测细胞管腔形成的情况,用实时定量RT-PCR和Western-Blot检测细胞中血管内皮生长因子mRNA和蛋白的表达情况。结果：在正常和高糖条件下,人参皂甙Rg3对于人视网膜血管内皮细胞增殖、细胞迁徙和细胞内皮管腔形成均具有抑制作用,且呈浓度与时间依赖性;且人参皂甙Rg3具有抑制人视网膜血管内皮细胞VEGF蛋白和mRNA表达的作用。结论：通过抑制VEGF的表达,人参皂甙Rg3可抑制人视网膜血管内皮细胞增殖、迁徙和管腔形成,进而抑制新生血管的形成。
Bibliography:AIM： To investigate the angiogenesis effect and mechanism of ginsenoside Rg3 on human retinal capillary endothelial cells （HRCECs） cultured in normal and high glucose conditions. METHODS： We cultured HRCEC both in normal and high glucose conditions. Each condition we treated the cells with 0. lmmoL/L ginsenoside Rg3 and 0.5mmoL/L ginsenoside Rg3. The effect of ginsenoside Rg3 on HRCEC proliferation was tested by methylthiazoletrazolium （MTT） assay 24h, 48h and 72h after the treatment; The effect of cell migration was tested by transwell; The effect of tube formation was tested by Matrigel; Western-Blot and real-time quantitative RT-PCR were used to detect the expression of vascular endothelial growth factor （VEGF） protein and mRNA. Data were statistically analyzed by ANOVA method. RESULTS： Ginsenoside Rg3 could inhibit proliferation, migration and tube formation of HRCEC in both conditions, depending on the concentration and time. Ginsenoside Rg3 could decrease the expression of VEGF mRNA and protein in both
ISSN:1672-5123
DOI:10.3980/j.issn.1672-5123.2013.10.06