肺炎支原体肺炎患儿肺炎支原体耐药性与DNA载量和基因型的关系研究

肺炎支原体肺炎患儿肺炎支原体耐药性与DNA载量和基因型的关系研究

目的探讨肺炎支原体（MP）肺炎患儿MP耐药情况及其与DNA载量和基因型的关系。方法选取2012年1月至2016年12月诊断为MP肺炎的230例住院患儿为研究对象,采集所有患儿咽拭子标本,采用快速培养基培养药敏法测定9种常用抗菌药物对MP临床分离株的药物敏感性;荧光实时定量PCR检测患儿咽拭子MP-DNA载量;PCR测序检测MP 23S r RNA V区2063位基因型。结果 230例MP患儿中,86例2063位基因型为A（37.4%）,134例为G（58.3%）,8例为C（3.5%）,2例为T（0.9%）。突变基因型（G＋C＋T）MP-DNA载量高于野生基因型（A）菌株（P〈0.05）;红霉素...

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Bibliographic Details
Published in:中国当代儿科杂志 Vol. 19; no. 11; pp. 1180 - 1184
Main Author: 张慧芬 白海涛 李基明 谢辉 王烨
Format: Journal Article
Language:Chinese
Published: 2017
Subjects:
DNA载量
儿童
基因型
耐药性
肺炎支原体
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Summary:目的探讨肺炎支原体（MP）肺炎患儿MP耐药情况及其与DNA载量和基因型的关系。方法选取2012年1月至2016年12月诊断为MP肺炎的230例住院患儿为研究对象,采集所有患儿咽拭子标本,采用快速培养基培养药敏法测定9种常用抗菌药物对MP临床分离株的药物敏感性;荧光实时定量PCR检测患儿咽拭子MP-DNA载量;PCR测序检测MP 23S r RNA V区2063位基因型。结果 230例MP患儿中,86例2063位基因型为A（37.4%）,134例为G（58.3%）,8例为C（3.5%）,2例为T（0.9%）。突变基因型（G＋C＋T）MP-DNA载量高于野生基因型（A）菌株（P〈0.05）;红霉素、阿奇霉素、克拉霉素、克林霉素耐药组MP-DNA载量高于非耐药组（P〈0.05）。MP对大环内酯类抗生素耐药率较高,60%以上产生大环内酯类耐药的病例均检测出A2063G突变,喹诺酮类药物少见MP耐药（低于2%）。结论 23S r RNA V区2063位点发生基因突变可能导致MP对大环内酯类药物耐药和DNA载量的变化,可作为MP治疗用药的选择依据。
Bibliography:Mycoplasma pneumoniae; Drug resistance; DNA load; Genotype; Child
ZHANG Hui-Fen, BAI Hai-Tao, LI Ji-Ming, XIE Hui, WANG Ye. （ Department of Pediatrics, Xiamen Maternal and Child Health Care Hospital, Xiamen, Fujian 361003, China）
43-1301/R
Objective To investigate the association of drug resistance of Mycoplasma pneumoniae（MP） with DNA load and genotypes in children with MP pneumonia.Methods A total of 230 children who were hospitalized and diagnosed with MP pneumonia between January 2012 and December 2016 were enrolled.Throat swabs were collected from the 230 children,and a rapid drug sensitivity assay was used to determine the sensitivity of clinical isolates of MP to nine commonly used antibacterial agents.Quantitative real-time PCR was used to measure MP-DNA load in throat swabs.PCR sequencing was used to determine the genotype of 2063 locus of the MP 23 S r RNA V domain.Results Of the 230 children,86（37.4%） had genotype A in 2063 locus,134（58.3%） had genotype G,8（3.5%） had genotype C,and 2（0.9%） had genotyp
ISSN:1008-8830