Search Results - "岩田, 祐之"

エクアドルのリーシュマニア症流行地におけるサシチョウバエの遺伝子タイピング法の確立 by 寺山, 好美, 加藤, 大智, Gomez, E.A, 上里, 博, Calvopina, M, 岩田, 祐之, 橋口, 義久

“…リーシュマニア症を媒介するサシチョウバエの簡便な種の鑑別法を確立することを目的とし、PCR-RFLP法を用いた遺伝子タイピング法の開発を行った。エクアドルのリーシュマニア症流行地9地点でサシチョウバエを採取し、形態学的に種を同定した。得られた7種のサシチョウバエからDNAを抽出、PCRにより18S…”
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Expression of porcine interleukin-2 in Escherichia coli by Iwata, H. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Yamamoto, M, Hasegawa, A, Kurata, K, Inoue, T

“…A mature form of porcine interleukin−2(IL−2)protein without signal peptides was expressed as glutathione S−transferase(GST)fusion proteins in Escherichia coli…”
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Fusobacterium necrophorum実験感染マウスでの遅延型過敏症 by 井上, 武, 中小路, 宗俊, 岩田, 祐之

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マウスの乳酸脱水素酵素-5クリアランスに対する部分肝切除の効果(短報) by 阿部, 冬樹, 林, 俊春, 岩田, 祐之, 井上, 武

“…ICRマウスにおける肝臓の乳酸脱水素酵素-5(LDH-5)クリアランス能を部分肝切除により検討した. 30もしくは50%の部分肝切除の場合, LDHクリアランス能はSham群と比較して低下する傾向がみられ, 65%除去では有意に低下した. また, 血中のLDH活性は50および65%の肝切除で有意に上昇したが,…”
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豚腸管膜リンパ節細胞におけるIL-2活性 by 岩田, 祐之, 上田, 竜夫, 高柳, 恭子, 和田, 睦美, 井上, 武

“…豚のインターロイキン2(IL-2)活性をマウスIL-2依存性細胞株(CTLL-2)を用いてMTT比色定量法により測定した. PHAで刺激した豚の腸管膜リンパ節(MLN)細胞の培養上清中にはCTLL-2細胞を濃度依存性に増殖させるIL-2活性が認められた. また, 1～2×107…”
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比色定量法による豚リンパ球幼若化反応の測定(短報) by 岩田, 祐之, 井上, 武

“…Phytohemagglutinin-P(PHA)およびconcanavalin A(ConA)による豚リンパ球の幼若化反応を3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) を用いた比色定量法で測定し,…”
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Fusobacterium necrophorum実験感染マウスでの遅延型過敏症 by 井上, 武, 中小路, 宗俊, 岩田, 祐之

“…Fusobacterium necrophorum実験感染マウスにおける遅延型過敏症の誘導を知るために足蹠反応を試みた. F. necrophorum生菌のマウス腹腔内投与により感作を行った. 本反応では感作後足蹠反応を惹起するには同一菌の生菌より加熱死菌のほうが良好である…”
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臨床病理学:豚インターロイキン2の大腸菌発現(短報) by 岩田, 祐之, 山本, 真理, 長谷川, 温彦, 倉田, 健, 井上, 武

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Molecular typing of sand fly species (Diptera, Psychodidae, Phlebotominae) from areas endemic for leishmaniasis in Ecuador by PCR-RFLP of 18S ribosomal RNA gene by Terayama, Y.(Yamaguchi Univ. (Japan). Faculty of Agriculture), Kato, H, Gomez, E.A, Uezato, H, Calvopina, M, Iwata, H, Hashiguchi, Y

“…Surveillance of the distribution of sand fly species is important for prediction of the risk and expansion of Leishmania infection in endemic and surrounding…”
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Gene expression changes induced by type IV allergy-inducible chemicals in dendritic cells by Tarama, R.(Yamaguchi Univ. (Japan). Faculty of Agriculture), Kato, H, Ishikawa, Y, Miyaura, H, Takeyoshi, M, Iwata, H

“…In the present study, the changes of gene expression profile in dendritic cell (DC)-derived DC2.4 cells sensitized with two allergenic chemicals were analyzed…”
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Development of a monoclonal antibody-based sandwich ELISA for detection of guinea pig interleukin-2 by Adachi, S.(Yamaguchi Univ. (Japan). Faculty of Agriculture), Hashimoto, T, Takeyoshi, M, Kato, H, Iwata, H

“…Interleukin 2 (IL-2) is a T cell proliferation factor released by Th0- and Th1-type helper T cells and is an essential cytokine for immune responses. In the…”
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Enzyme-linked immunosorbent assay for the detection of canine leptospira antibodies using recombinant OmpL1 protein by Okuda, M. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Sakai, Y, Matsuuchi, M, Oikawa, T, Watanabe, M, Itamoto, K, Iwata, H, Kano, R, Hasegawa, A, Onishi, T, Inokuma, H

“…OmpL1 is a 31-kDa outer membrane protein characterized in 1993 and known to be expressed only in pathogenic Leptospira spp. Recombinant OmpL1 (OST-rOmpL1) was…”
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Centrosome amplification and chromosomal instability in feline lymphoma cell lines by Miki, R. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Okuda, M, Oikawa, T, Watanabe, M, Ma, Z, Matsumoto, K, Iwata, H, Inokuma, H

“…To evaluate the presence of centrosome amplification and the resulting chromosomal instability in cat tumors, a newly established feline lymphoma cell line and…”
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Production and characterization of monoclonal antibodies against porcine interleukin-4 by Ishikura, Y. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Kato, H, Hashimoto, T, Nishimura, Y, Iwata, H

“…Interleukin 4 (IL-4) is an important regulatory cytokine produced by activated T lymphocytes and mast cells, and regulates the growth and differentiation of…”
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Generation of monoclonal antibodies to porcine interleukin 6 (PIL-6) using the recombinant PIL-6 expressed in Escherichia coli by Watanabe, R. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Hasegawa, A, Miyazawa, T, Kato, H, Iwata, H

“…Porcine interleukin-6 (PIL-6) protein without signal peptide was expressed as a glutathione S-transferase (GST) fusion protein in Escherichia coli. The fusion…”
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The complete nucleotide sequences of L3 and S7 segments of Ibaraki virus encoding for the major inner capsid proteins, VP3 and VP7 by Iwata, H. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Manabe, S, Yoshida, A, Pereira, E.M.N, Inoue, T

“…The complete nucleotide sequences of the genes encoding two of the major inner capsid proteins of Ibaraki virus (IBAV), belonging to epizootic hemorrhagic…”
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Expression of recombinant feline serum amyloid A (SAA) protein by Ohno, K. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Terado, M, Iwata, H, Inokuma, H, Onishi, T

“…Feline serum amyloid A (SAA) cDNA clone was isolated from a hepatic mRNA of a mixed-breed cat. The feline SAA cDNA clone contains 333 nucleotides and deduced…”
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Expression of bovine cytokines in Escherichia coli by Kashima, T. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Morishita, A, Iwata, H, Maeda, K, Inoue, T

“…Glutathione S-transferase fusion proteins of bovine interleukin-2 (IL-2), IL-4, IL-6 and interferon-gamma (IFN-gamma) were expressed in Escherichia coli…”
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Expression of Recombinant Bovine L-Selectin in Escherichia coli and Insect Cells by Kashima, Takayuki, Hasegawa, Atsuhiko, Iwata, Hiroyuki, Inoue, Takeshi

“…Bovine L-selectin was expressed in bacteria using pGEX vector and in insect cells infected with recombinant baculovirus in order to obtain recombinant protein…”
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Expression of bovine interleukin-2 in insect cells by recombinant baculovirus by Kashima, T. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Iwata, H, Inoue, T

“…Bovine interleukin-2 (bIL-2) was expressed in insect cells infected with recombinant baculovirus. The cDNA for bIL-2 was amplified by reverse transcriptional…”
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