The role of calcium in endotoxin-induced release of calcitonin gene-related peptide (CGRP) from rat spinal cord
In the present study, the role of calcium in endotoxin-induced CGRP release was studied. 2 .5-50 μg/mL endotoxin and 1 -10 mmol/L caffeine caused concentration-dependent increase of CGRP release from rat spinal cord in vitro. However, no additive effect could he found when caffeine and endotoxin wer...
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| Published in: | Science China. Life sciences Vol. 40; no. 4; pp. 437 - 442 |
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| Main Author: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
China
Springer Nature B.V
01-08-1997
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | In the present study, the role of calcium in endotoxin-induced CGRP release was studied. 2 .5-50 μg/mL endotoxin and 1 -10 mmol/L caffeine caused concentration-dependent increase of CGRP release from rat spinal cord in vitro. However, no additive effect could he found when caffeine and endotoxin were concomitantly incubated. By using capsaicin, Ca2+-free medium, Omega-Conotoxin, nifedipine, W-7, ryanodine, MgCl2, Tris-ATP, rutheni-um red, the results indicate that the release of CGRP evoked by endotoxin from the sensory fibers of rat spinal cord is dependent on extracellular calcium. After entering into the cell through the N-type calcium channel, calcium binds to calmodulin, and triggers calcium release from intracellular calcium store by activating the caffeine-sensitive but ryan-odine-insensitive mechanism. |
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| Bibliography: | 11-5841/Q TANG Yueming HAN Qide and WANG Xian(Institute of Vascular Medicine, The Third Hospital, Beijing Medical University, Beijing 100083, China) ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
| ISSN: | 1674-7305 1006-9305 1869-1889 1862-2798 |
| DOI: | 10.1007/BF02881739 |