Transcriptome of intraperitoneal organs of starry flounder Platichthys stellatus challenged by Edwardsiella ictaluri JCM1680

Platichthys stellatus is an economically important marine bony fish species that is cultured in China on a large scale. However, very little is known about its immune-related genes. In this study, the transcriptome of the immune organs ofP. stellatus that were intraperitoneally challenged with the p...

Full description

Saved in:

Bibliographic Details
Published in:	Chinese journal of oceanology and limnology Vol. 33; no. 1; pp. 20 - 27
Main Author:	仝颜丽孙修勤王波王玲李妍田金虎郑凤荣郑明刚
Format:	Journal Article
Language:	English
Published:	Heidelberg Springer-Verlag 01-01-2015 Science Press Springer Nature B.V
Subjects:	Amino acid sequence Aquaculture Biology Body organs Chemokines Earth and Environmental Science Earth Sciences Economic importance Edwardsiella ictaluri Fish Fish culture flounder gene expression regulation Genes immune response Immunity immunomodulation Immunoregulation Interleukin 6 Intestine Intestines kidneys liver Major histocompatibility complex Marine Marine fish Marine fishes Necrosis Nucleotide sequence Oceanography Organs Pathogens PCR Platichthys stellatus Pleuronectiformes Polymerase chain reaction Protein sequencing quantitative polymerase chain reaction Receptors RNA sequence analysis Spleen tissues transcriptome Transcriptomes Transcriptomics Tumor necrosis factor Tumor necrosis factor receptors tumor necrosis factors unigenes 主要组织相容性复合物免疫器官实时定量PCR 比目鱼白细胞介素-6 腹腔蛋白质序列数据库转录水平 China China, People's Rep illumina sequencing immunity gene transcriptome real-time quantitative PCR
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Platichthys stellatus is an economically important marine bony fish species that is cultured in China on a large scale. However, very little is known about its immune-related genes. In this study, the transcriptome of the immune organs ofP. stellatus that were intraperitoneally challenged with the pathogen Edwardsiella ictaluri JCM1680 is analyzed. Total RNA from four tissues （spleen, kidney, liver, and intestine） was mixed equally and then sequenced on an Illumina HiSeq 2000 platform. Overall, 28 465 813 quality reads were generated and assembled into 43 061 unigenes. Similarity searches against public protein sequence databases were used to annotate 28 291 unigenes （65.7% of the total）, 368 of which were associated with immunoregulation, including 188 related to immunity response. Additionally, the transcript levels of immunity response unigenes annotated as related to tumor necrosis factor （TNF）, TNF receptor, chemokine, major histocompatibility complex, and interleukin-6 were investigated in the different tissues of normal and infected P. stellatus by real-time quantitative PCR. The results confirmed that the unigenes identified in the transcriptome database were indeed expressed and up-regulated in infected P. stellatus. To our knowledge, this is the first report of the sequencing and analysis of the transcriptome of P. stellatus. These findings provide insights into the transcriptomics and immunogenetics of bony fish.
Bibliography:	Platichthys stellatus; illumina sequencing; transcriptome; immunity gene; real-time quantitativePCR TONG Yanli , SUN Xiuqin , WANG Bo , WANG Ling , LI Yan , TIAN Jinhu , ZHENG Fengrong, ZHENG Minggang （1.College of Marine Life Science, Ocean University of China Qingdao 266003, China; 2.Research Center for Marine Ecology, First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China ;3. College of Chemistry and Chemical Engineering and Environment, Qingdao University, Qingdao 266071, China） Platichthys stellatus is an economically important marine bony fish species that is cultured in China on a large scale. However, very little is known about its immune-related genes. In this study, the transcriptome of the immune organs ofP. stellatus that were intraperitoneally challenged with the pathogen Edwardsiella ictaluri JCM1680 is analyzed. Total RNA from four tissues （spleen, kidney, liver, and intestine） was mixed equally and then sequenced on an Illumina HiSeq 2000 platform. Overall, 28 465 813 quality reads were generated and assembled into 43 061 unigenes. Similarity searches against public protein sequence databases were used to annotate 28 291 unigenes （65.7% of the total）, 368 of which were associated with immunoregulation, including 188 related to immunity response. Additionally, the transcript levels of immunity response unigenes annotated as related to tumor necrosis factor （TNF）, TNF receptor, chemokine, major histocompatibility complex, and interleukin-6 were investigated in the different tissues of normal and infected P. stellatus by real-time quantitative PCR. The results confirmed that the unigenes identified in the transcriptome database were indeed expressed and up-regulated in infected P. stellatus. To our knowledge, this is the first report of the sequencing and analysis of the transcriptome of P. stellatus. These findings provide insights into the transcriptomics and immunogenetics of bony fish. 37-1150/P http://dx.doi.org/10.1007/s00343-015-3307-7 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0254-4059 2096-5508 1993-5005 2523-3521
DOI:	10.1007/s00343-015-3307-7