Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

This study aims to determine the diversity of culturable thermophilic bacteria isolated from eight terrestrial hot springs in Northeastern of Algeria using the conventional methods, SDS-PAGE fingerprinting of whole-cell proteins and 16S rRNA gene sequencing. In addition, their hydrolytic enzyme acti...

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Bibliographic Details
Published in:	Brazilian journal of microbiology Vol. 51; no. 4; pp. 1987 - 2007
Main Authors:	Benammar, L., İnan Bektaş, K., Menasria, T., Beldüz, A. O., Güler, H. I., Bedaida, I. K., Gonzalez, J. M., Ayachi, A.
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-12-2020
Subjects:	Biomedical and Life Sciences Environmental Microbiology - Research Paper Food Microbiology Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Mycology Thermal spring Thermozyme Culture-dependent method Thermophile Algeria Biodiversity SDS-PAGE fingerprinting 16S rRNA gene
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Summary:	This study aims to determine the diversity of culturable thermophilic bacteria isolated from eight terrestrial hot springs in Northeastern of Algeria using the conventional methods, SDS-PAGE fingerprinting of whole-cell proteins and 16S rRNA gene sequencing. In addition, their hydrolytic enzyme activities were also investigated. A total of 293 strains were isolated from the hot springs’ water and sediment using different culture media. Overall, five distinct bacterial groups were characterized by whole-cell protein pattern analysis. Based on the 16S rRNA gene sequencing of 100 selected strains, the isolates were assigned to the following three major phyla: Firmicutes (93%), Deinococcus-Thermus (5%), and Actinobacteria (2%), which included 27 distinct species belonging to 12 different phylotypes, Aeribacillus , Aneurinibacillus , Anoxybacillus , Bacillus , Brevibacillus , Geobacillus , Laceyella , Meiothermus , Saccharomonospora , Thermoactinomyces , Thermobifida , and Thermus . The screening for nine extracellular enzymes showed that 65.87% of the isolates presented at least five types of enzyme activities, and 6.48% of strains combined all tested enzymes (amylase, cellulase, pectinase, esculinase, protease, gelatinase, lipase, lecithinase, and nuclease) . It was found that Bacillus , Anoxybacillus , Aeribacillus , and Aneurinibacillus were the genera showing the highest activities. Likewise, the study showed an abundant and diverse thermophilic community with novel taxa presenting a promising source of thermozymes with important biotechnological applications. This study showed that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully differentiate thermophilic bacteria from Algerian hot springs.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Responsible Editor: Vania M.M. Melo
ISSN:	1517-8382 1678-4405
DOI:	10.1007/s42770-020-00376-0