PER-1- and OXA-10-like β-lactamases in ceftazidime-resistant Pseudomonas aeruginosa isolates from intensive care unit patients in Istanbul, Turkey

The presence of PER-1- and OXA-10-like β-lactamases was investigated by PCR in 49 ceftazidimeresistant Pseudomonas aeruginosa isolates from patients hospitalised in the 24-bed general intensive care unit of the Istanbul Faculty of Medicine during a 12-month period between February 1999 and February...

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Published in:	Clinical microbiology and infection Vol. 11; no. 3; pp. 193 - 198
Main Authors:	Aktaş, Z., Poirel, L., şalcloğlu, M., özcan, P.E., Midilli, K., Bal, ç., Anğ, ö., Nordmann, P.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Elsevier Ltd 01-03-2005 Blackwell Science Ltd Blackwell
Subjects:	Anti-Bacterial Agents - pharmacology Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents beta-Lactamases - metabolism Biological and medical sciences Ceftazidime - pharmacology ceftazidime resistance Cephalosporin Resistance - physiology Humans intensive care unit Intensive Care Units Medical sciences OXA-10 PER-1 Pharmacology. Drug treatments Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - enzymology Pseudomonas Infections - drug therapy Pseudomonas Infections - microbiology Turkey β-Lactamase Asia Turkey OXA-10 Pseudomonas aeruginosa intensive care unit β-Lactamase ceftazidime resistance PER-1 Pseudomonadales Prevalence Cefepime Epidemiology Intensive care unit Penicillin derivatives Carbapenem derivatives Gene Piperacillin Bacteria Pseudomonadaceae Activity spectrum Ceftazidime Human Nucleotide sequence Enzyme β-Lactams Enzyme inhibitor Monobactam derivatives Random amplified polymorphic DNA marker Infection Polymerase chain reaction Resistance Cephalosporin derivatives Antibiotic Tazobactam Azetidine derivatives Aztreonam Hydrolases Molecular biology Antibacterial agent Imipenem
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Summary:	The presence of PER-1- and OXA-10-like β-lactamases was investigated by PCR in 49 ceftazidimeresistant Pseudomonas aeruginosa isolates from patients hospitalised in the 24-bed general intensive care unit of the Istanbul Faculty of Medicine during a 12-month period between February 1999 and February 2000. The clonal relatedness of the isolates was investigated by random amplified polymorphic DNA (RAPD) analysis, and the sequences of the PER-1 and OXA genes from all isolates were determined. The rates of resistance of the isolates to imipenem, aztreonam and cefepime were 98%, 92% and 96%, respectively, and to piperacillin and piperacillin–tazobactam were 41%and 37%, respectively. Using the double-disk synergy test, 37% (18/49) of the isolates were identified as extended-spectrum β-lactamase producers. The PER-1 gene was identified in 86% (42/49) and the OXA-10 gene in 55% (27/49) of the ceftazidime-resistant isolates. Of isolates carrying the PER-1 gene, 48% (20/42) also carried the OXA-10 gene. The respective nucleotide sequences were identical for each isolate. Sixteen RAPD patterns were detected among the PER-1-positive isolates, but 60% (25/42) of the PER-1-positive isolates belonged to two distinct patterns, while the remainder exhibited a wide clonal diversity. The results indicated that the prevalence of PER-1- and OXA-10-like β-lactamases remains high among ceftazidime-resistant P. aeruginosa isolates in Turkey.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1198-743X 1469-0691
DOI:	10.1111/j.1469-0691.2004.01067.x