Human genomic DNA isolation from whole blood using a simple microfluidic system with silica- and polymer-based stationary phases

Monodisperse-porous silica microspheres 5.1μm in size with a bimodal pore-size distribution (including both mesoporous and macroporous compartments) were obtained using a newly developed staged-shape templated hydrolysis and condensation protocol. Synthesized silica microspheres and monodisperse-por...

Full description

Saved in:

Bibliographic Details
Published in:	Materials Science & Engineering C Vol. 74; pp. 10 - 20
Main Authors:	Günal, Gülçin, Kip, Çiğdem, Öğüt, Sevim Eda, Usta, Duygu Deniz, Şenlik, Erhan, Kibar, Güneş, Tuncel, Ali
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-05-2017 Elsevier BV
Subjects:	Adsorption Blood Chemical synthesis Compartments Condensation polymerization Deoxyribonucleic acid DNA DNA - blood DNA - isolation & purification DNA microextraction Genome, Human Humans Materials science Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microfluidics Microscopy, Electron, Scanning Microspheres Packing Particle Size Permeability Polymerization Polymers - chemistry Pore size Pore size distribution Porosity Porous particles Silica Silicon dioxide Silicon Dioxide - chemistry Size distribution Slurries Sorbent Sorbents Yield DNA microextraction Adsorption Sorbent Porous particles
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Monodisperse-porous silica microspheres 5.1μm in size with a bimodal pore-size distribution (including both mesoporous and macroporous compartments) were obtained using a newly developed staged-shape templated hydrolysis and condensation protocol. Synthesized silica microspheres and monodisperse-porous polymer-based microspheres with different functionalities, synthesized by staged-shape template polymerization, were comparatively tested as sorbents for human genomic DNA (hgDNA) isolation in a microfluidic system. Microcolumns with a permeability range of 1.8–8.5×10−13m2 were fabricated by the slurry-packing of silica- or polymer-based microspheres. The monodisperse-porous silica microspheres showed the best performance in hgDNA isolation in an aqueous buffer medium; >2500ng of hgDNA was recovered with an isolation yield of about 50%, using an hgDNA feed concentration of 100ng/μL. Monodisperse-porous silica microspheres were also evaluated as a sorbent for genomic DNA isolation from human whole blood in the microfluidic system; 14ng of hgDNA was obtained from 10μL of whole blood lysate with an isolation yield of 64%. Based on these results, we conclude that monodisperse-porous silica microspheres with a bimodal pore size distribution are a promising sorbent for the isolation of hgDNA in larger amounts and with higher yields compared to the sorbents previously tried in similar microfluidic systems. [Display omitted] •Monodisperse silica microspheres with bimodal pore size distribution as DNA sorbent•The presence of mesoporous and macroporous compartments in the porous structure•Genomic DNA isolation from human whole blood in the microfluidic system•Superior DNA isolation performance with respect to currently available sorbents
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0928-4931 1873-0191
DOI:	10.1016/j.msec.2016.12.118