Macro and micro-structural study on Aspergillus parasiticus inoculated in peanut kernels treated with gamma radiation (Cs137)

Macro and micro-structural study on Aspergillus parasiticus inoculated in peanut kernels treated with gamma radiation (Cs137)

This study investigated the micro and macro-morphology of Aspergillus parasiticus, CMT 00064, inoculated into peanut kernels, after gamma irradiation with Cs137. Plates containing kernels inoculated with the fungal strain were incubated in a BOD germination chamber at 25oC for 5 days. These plates w...

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Bibliographic Details
Published in:Journal of Bioenergy and Food Science Vol. 4; no. 1; pp. 68 - 77
Main Authors: Valéria Barbosa Borges, Maria Antonieta Peixoto Gimenes Couto, Mauro Carlos Lopes Souza, Áurea Maria Lages de Moraes, Marta Maria Braga Baptista Soares Xavier
Format: Journal Article
Language:English
Published: Instituto Federal de Educação, Ciência e Tecnologia do Amapá 01-04-2017
Subjects:
Aspergillus parasiticus
Cesium 137
Food Irradiation
Micro and macro-morphology
Peanuts
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Summary:This study investigated the micro and macro-morphology of Aspergillus parasiticus, CMT 00064, inoculated into peanut kernels, after gamma irradiation with Cs137. Plates containing kernels inoculated with the fungal strain were incubated in a BOD germination chamber at 25oC for 5 days. These plates were irradiated at the dose rate of 1.6 kGy/min on the sixth day of incubation. The absorbed doses were: 0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0 kGy. The observed inactivation doses ranged from 4.5 to 8.0 kGy between days 1, 7 and 15 after irradiation. Fungus growth was observed 15 days after irradiation in the plates irradiated between 0 and 7.5 kGy demonstrating that the dose of 8.0 kGy could eliminate the fungus. Subcultures in nutrient media of isolates irradiated at 7.5 kGy recovered its growth in 15 days. Kernels irradiated with 6.5 kGy grew without metula indicating elimination. Colonies that grown after irradiation up 6.0 kGy were biseriate. However, after irradiation with 6.5 kGy they were uniseriate. The survival rates of A. parasiticus, in irradiated substrate peanuts, decreases with increasing absorbing doses of ionizing radiation.
ISSN:2359-2710
DOI:10.18067/jbfs.v4i1.127